Hepatitis

CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 (CRISPR associated protein 9) system is adapted from a naturally occurring bacterial immune system that can protect bacteria from damage caused by phage infection. As an unimagined tool for genome engineering, CRISPR/Cas9 is now commonly used. Compared with ZFNs and TALENs, CRISPR/Cas9 employs sgRNA (single guide RNA) to specify editing which makes the system more precise, time/cost-saving and quite valuable for high-throughput genome editing.

Creative Biogene has developed a series of Cas9 expressing stable cell lines. The Cas9 encoding gene is stably integrated into either random site, or AAVS1 safe harbor site of human genome, or ROSA26 safe harbor site of mouse genome. Apart from HEK293 and HeLa cells, we have achieved Cas9 stable expression in several types of cancer cells which makes it convenient for scientists to study related gene functions of certain cancers. For consistent, high-level and more stable expression of Cas9, monoclonal cells are isolated. The activity of Cas9 in each constructed stable cell line is functionally validated by the means of T7 Endonuclease I assay.