This training introduces the clinical use of next-generation sequencing (NGS) for HIV drug-resistance testing, with a focus on bioinformatics analysis and result interpretation.

Participants will learn why drug-resistance testing is important in clinical care, how the automated Nextflow pipeline supports the analysis, and how to interpret the results. Through hands-on exercises, participants will learn how to retrieve the latest HIVdb database using sierra-local, set up and run the nf-core/viralrecon pipeline on Illumina sequencing data, and configure the computational resources required for the analysis.

 Dates: Tuesday 1 and Tuesday 8 December 2026. 

 Duration: Two half-days 09:00-12:30 CET. 

Location: Online - Find the link after enrolment. 

Audience: The intended audience are bioinformaticians, microbiologists, molecular biologists or public health professionals with some computational experience.

Objectives - Upon completing this course, participants will be able to:

• Have a general understanding of performing NGS-bases HIV drug-resistance testing in routine care;
• Be able to set up and run sierra-local and retrieve the latest HIV drug-resistance database; 
• Be able to set up and run nf-core/viralrecon pipeline locally or on a cluster;
• Be able to configure the computation resources for running the pipeline;
• Have a good understanding of how to interpret and report the results to the treating physician.

Participation: This course is open for public enrolment.